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Density Gradient Processing

California Cryobank uses density gradient processing to prepare IUI ready donor specimens. While more costly and time consuming, density gradient processing is superior to other methods.

Density gradient processing:

  • Removes cells that are associated with bacteria, viruses and infection. (1)
  • Pre-selects sperm that have superior motility. (2)
  • Pre-selects sperm with a lower incidence of DNA anomalies. (3)
  • Removes seminal plasma, thus reducing post insemination cramping.

If you would like more information or have questions about the density gradient processing method, please call our laboratory manager at (310) 443-5244, x1165.

(1) Hans Wolff. The biological significance of white blood cells in semen. Fert. & Ster. Vol. 63, No. 6, June 1995.
(2) Smith, S. et al. Use of postpreparation sperm parameters to determine the method of choice for assisted reproductive technology. Fert. & Ster. 1995; 63:591-597.
(3) Sakkas et al. The use of two dense degraded centrifugation techniques and swim-up sperm separation to separate spermatazoa with chromatin and nuclear DNA anomalies. Human Reprod. In press.

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National averages range from 3-4 insemination cycles per successful pregnancy. We recommend purchasing at least 5 vials per child to guarantee your donor remains available until reaching your personal family goals. Additionally, you get 1 Year FREE storage if you purchase 4 or more vials ($475 value).


Our donors sell out and retire quickly! We have thousands of clients forced to choose between a new donor or not having a second child because they chose not to store vials for future use while they were available. To help you plan for your future, we offer 3 Years FREE storage if you purchase 3 Years+ vials ($1,050 value) - as well as the ability to sell back any unused vials that have not left our control.